MSC Researcher / Project & Data Support — University of Sussex (SRSF4 localization imaging and quantitative image analysis)
You performed quantitative analysis of immunofluorescence microscopy data to quantify subcellular localization signals under ER stress. You used nuclear speckle vs cytoplasmic distribution metrics to classify and interpret localization changes from experimental images. This work required careful per-sample image processing and consistent labeling of regions of interest across conditions. • Used confocal microscopy channels (SC35/Alexa Fluor 488, SRSF4/Cy3, DAPI) for image-based evidence. • Computed Pearson correlation and Manders coefficient for localization quantification. • Applied ImageJ with JaCoP tools to support the image analysis workflow. • Interpreted results focused on nuclear speckles vs cytoplasmic localization shifts.